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The traditional microscope is combined with the camera system, display or computer to achieve the purpose of magnifying the object to be measured. The earliest prototype should be a camera type microscope. The image obtained under the microscope is projected onto a photosensitive photograph through the principle of pinhole imaging to obtain a picture. Or directly connect the camera with the microscope to take pictures.
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Because the dark field microscope does not project transparent light into the direct observation system, when there is no object, the field of vision is dark, and it is impossible to observe any object. When there is an object, the light diffracted by the object and the scattered light are bright and visible in the dark background. When observing an object in the dark field, most of the illumination light is turned back. Due to the position, structure and thickness of the object (specimen), the scattering and refraction of light have great changes.
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It was first created by Jensen and his son in the Netherlands in 1590. The optical microscope can magnify the object 1600 times, and the minimum resolution limit is 0.1 μ m. There are many kinds of optical microscopes.
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Digital microscope is a high-tech product developed by combining the excellent optical microscope technology, advanced photoelectric conversion technology and liquid crystal screen technology. Thus, we can study the micro field from the traditional ordinary binocular observation to the reproduction on the display, thus improving the work efficiency.
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Scanning tunneling microscope, also known as "scanning tunneling microscope" and "tunnel scanning microscope", is an instrument that uses the tunneling effect in quantum theory to detect the surface structure of materials. It was invented by G. Binning and H. Rohrer in IBM's Zurich laboratory in Zurich, Switzerland, in 1981. Therefore, the two inventors shared the 1986 Nobel Prize in Physics with Ernst Ruska.
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Portable microscope is mainly an extension of digital microscope and video microscope series developed in recent years. Different from traditional optical amplification, hand-held microscopes are digital amplification, which generally pursue portability, small and delicate, easy to carry; Moreover, some hand-held microscopes have their own screens, which can be imaged independently from the computer host, and are easy to operate.
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The electron microscope has the basic structural characteristics similar to the optical microscope, but it has much higher magnification and resolution ability for objects than the optical microscope. It takes the electron flow as a new light source to make objects image. Since Ruska invented the first transmission electron microscope in 1938, in addition to the continuous improvement of the performance of the transmission electron microscope, other types of electron microscopes have also been developed.
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The desk microscope, mainly referring to the traditional microscope, is a pure optical magnification with high magnification and good imaging quality. However, it is generally large and inconvenient to move. It is mostly used in the laboratory and inconvenient to go out or conduct on-site inspection.
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It usually consists of optical part, lighting part and mechanical part. Undoubtedly, the optical part is the most critical part, which consists of eyepiece and objective lens. As early as 1590, eyewear manufacturers in Holland and Italy had built magnifying instruments similar to microscopes. There are many kinds of optical microscopes, mainly including light field microscope (ordinary optical microscope), dark field microscope, fluorescence microscope, phase contrast microscope, laser scanning confocal microscope, polarizing microscope, differential interference difference microscope and inversion microscope.
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Polarizing microscope is a kind of microscope used to study so-called transparent and opaque anisotropic materials. It has important applications in science and engineering majors such as geology. All materials with birefringence can be clearly distinguished under a polarizing microscope. Of course, these materials can also be observed by staining method, but some are not. Instead, a polarizing microscope must be used. Reflective polarizing microscope is a necessary instrument for the research and identification of birefringent materials by using the polarization characteristics of light. It can be used for single polarized light observation, orthogonal polarized light observation, and cone light observation.
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